(Solution) 108 Bp Fraction Of Single Copy DNA That Hybridizes With Pollen MRNA At Saturation = 0.02 Calculate The Complexity Of Pollen MRNA From The Data Given... | Snapessays.com


(Solution) 108 bp Fraction of single copy DNA that hybridizes with pollen mRNA at saturation = 0.02 Calculate the complexity of pollen mRNA from the data given...


question about DNA gel plot Southern hybridization experiment and qRT-PCR experiment. (question is attached).3.

 

You have isolated a cDNA clone representing protein Z from leaves, obtain its nucleotide

 

sequence, and do two experiments.

 

First, you hybridize the cDNA clone with equal

 

amounts of nuclear DNA from leaves and roots in DNA gel blot (Southern) hybridization

 

experiments.

 

You also perform quantitativ e reverse transcription polymerase chain

 

reaction (qRT-PCR) experiments.

 

The following results are obtained.

 

DNA GEL BLOTS

 

L

 

R

 

a.

 

Is the Z gene differentially expressed during plant development?

 

Explain your

 

answer.

 

b.

 

Based on the results of the DNA gel blot hybridization experiments, suggest a

 

possible reason why the Z gene is or is not differentially expressed.

 

qRT-PCR Experiment

 

Organ

 

Relative mRNA Amount

 

Leaf

 

100

 

Root

 

10

 


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This question was answered on: May 23, 2022

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