(Solution) An experiment is carried out to monitor the kinetics of drug (D) binding to the receptor (R). > Snapessays.com


(Solution) An experiment is carried out to monitor the kinetics of drug (D) binding to the receptor (R).


1). An experiment is carried out to monitor the kinetics of drug (D) binding to the receptor (R). To monitor drug binding, the receptor is fluorescently labeled so that drug binding can be visualized as an increase in total fluorescence.  Experimental data for the rate of dissociation of drug from the receptor is provided below, where 10 nM of [RD] complex is allowed to dissociate over time.   Time (seconds) - [RD] complex (Fluorescence units) 0 -1500 2 -1305 4 -1245 6 -1170 8 -1095 10 -1035 12- 975 14- 930 A) Covert the fluorescence units into the concentration of [RD] complex remaining over time. To calibrate the assay, a 10 nM complex of drug bound to receptor [RD] is known to possess a total fluorescence value of 1500 units.How can in convert the fluorescence units into the concentration to solve for question A??

 


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This question was answered on: Sep 21, 2023

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