If there is a mutation that changes an A to a G in RNAII on the pColE1 plasmid, will RNAI still be able to inhibit it? Why or why not? (10 pts)2. You have discovered a new bacterial species where, during DNA replication, DNA primers are used instead of RNA primers. Why would this result be completely unexpected given our current understanding of biology and what you know about DNA replication? (10 pts)Given the processes we’ve talked about in class so far, describe a scenario whereby deletion of a single gene on the chromosome renders a plasmid essential for cell survival (turning that plasmid into a second chromosome)? (10pts)If you select for single base pair mutations which provide penicillin resistance to Bacillus anthracis, would you also expect that mutant to be resistant to vancomycin? Why or why not? (10pts)Imagine that you have set up an experiment to conjugate two strains: A) strain one is a methionine auxotroph, resistant to streptomycin, with this resistance gene located on a plasmid that closely resembles pColE1. B) Strain two is a methionine prototroph that contains no other plasmids. The polA gene has also been deleted from strain 2. Describe two independent reasons why this conjugation will fail (10 points)
This question was answered on: May 23, 2022
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